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3D Fourier transformation light scattering for reconstructing extend angled resolved light scattering of individual particles

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posted on 2023-01-11, 21:59 authored by Khoi Phuong Dao, Kyeoreh Lee, Yuri Hong, Seungwoo Shin, Sumin Lee, Dong Soo Hwang, Yongkeun Park
We represent three-dimensional Fourier transform light scattering, a method to reconstruct angle-resolved light scattering (ARLS) with extended angle-range from individual spherical objects. To overcome the angle limitation determined by the physical numerical aperture of an optical system, the optical light fields scattered from a sample are measured with various illumination angles, and then synthesized onto the Ewald Sphere corresponding to the normal illumination in Fourier space by rotating the scattered light signals. The method extends the angle range of the ARLS spectra beyond 90 degree, beyond the limit of forward optical measurements. Extended scattered light fields in 3D and corresponding ARLS spectra of individual microscopic polystyrene beads, and protein droplets are represented.

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