Complex Master-Slave Enhanced Optical Coherence Microscopy

We present an instrument designed to facilitate localization and high-resolution, Optical Coherence Microscopy (OCM) imaging of small biological samples immersed in a medium several orders of magnitude greater in volume. A modified turret-equipped microscope stand was inserted into the sample arm of a Spectral Domain Optical Coherence Microscopy (SD-OCM) system. The instrument enabled swift change of imaging objectives through the incorporation of Complex Master-Slave Interferometry (CMSI), providing tolerance to dispersion for any objective through the acquisition of a few (≥2) calibration spectra. We demonstrate the instrument's ability to localize and image samples by providing examples of its application to optical phantoms and to a porcine oocyte immersed in a biological culture medium.