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Estimation of the full shape of the crystalline lens from OCT: validation using stretched donor lenses

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posted on 2023-04-21, 10:44 authored by Eduardo Martinez-Enriquez, Bianca Maceo Heilman, Alberto de Castro, Ashik Mohamed, Marco Ruggeri, Fernando Zvietcovich, Fabrice Manns, Susana Marcos
Quantifying human crystalline lens geometry as a function of age and accommodation is important for improved cataract and presbyopia treatments. In previous works we presented eigenlenses as a basis of 3-D functions to represent the full shape of the crystalline lens ex vivo. Also, we presented the application of eigenlenses to estimate the full shape of the lens in vivo from 3-D optical coherence tomography (OCT) images, where only the central part of the lens -visible through the pupil- is available. The current work presents a validation of the use of eigenlenses to estimate in vivo the full shape of dis-accommodated lenses. We used 14 ex vivo crystalline lenses from donor eyes (11-54 y/o) mounted in a lens stretcher, and measured the geometry and the power of the lenses using a combined OCT and ray tracing aberrometry system. Ex vivo, the full extent of the lens is accessible from OCT because the incident light is not blocked by the iris. We measured in non-stretched (fully accommodated) and stretched (mimicking in vivo dis-accommodated lenses) conditions. Then, we simulated computationally in vivo conditions on the obtained ex vivo lenses geometry (assuming that just the portion of the lens within a given pupil is available), and estimated the full shape using eigenlenses. The mean absolute error (MAE) between estimated and measured lens’ diameters and volumes were MAE=0.26 ± 0.18 mm and MAE=7.0 ± 4.5 mm³, respectively. Furthermore, we concluded that the estimation error between measured and estimated lenses did not depend on the accommodative state (change in power due to stretching), and thus eigenlenses are also useful for the full shape estimation of in vivo dis-accommodated lenses.


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IMCUSTOMEYE H2020-ICT-2017; SILKEYE H2020-ERC-2018-ADG; Ministerio de Ciencia e Innovación; National Institutes of Health; Research to Prevent Blindness, Departamental Funds to Flaum Eye Institute and Bascom Palmer Eye Institute; National Institutes of Health; Unrestricted Funds Research to Prevent Blindness, NY; Florida Lions Eye Bank and Beauty of Sight Foundation; Hyderabad Eye Research Foundation; National Institutes of Health (R01EY021834; a National Institutes of Health Center Core Grant (P30EY014801)

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