posted on 2023-01-10, 03:12authored byTao Chen, Arindam Ghosh, Jörg Enderlein
Graphene-induced energy transfer (GIET) is a recently developed fluorescence-spectroscopic technique that achieves sub-nanometric optical localization of fluorophores along the optical axis of a microscope. GIET is based on the near-field energy transfer from an optically excited fluorescent molecule to a single sheet of graphene. It has been successfully used for estimating inter-leaflet distances of single lipid bilayers, and for investigating the membrane organization of living mitochondria. In this study, we use GIET to measure the cholesterol-induced subtle changes of membrane thickness at the nanoscale. We quantify membrane thickness variations in supported lipid bilayers (SLBs) as a function of lipid composition and increasing cholesterol content. Our findings demonstrate that GIET is an extremely sensitive tool for investigating nanometric structural changes in bio-membranes.
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