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Interface Self-Referenced Dynamic Full-Field Optical Coherence Tomography

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posted on 2023-02-21, 19:35 authored by Tual Monfort, Salvatore Azzollini, Tasnim Ben Yacoub, Isabelle Audo, Sacha Reichman, Kate Grieve, Olivier Thouvenin
Dynamic full-field optical coherence tomography (D-FFOCT) has recently emerged as an invaluable live label-free and non-invasive imaging modality able to image subcellular biological structures and their metabolic activity within complex 3D samples. However, D-FFOCT suffers from fringe artefacts when imaging nearby reflective surfaces and is highly sensitive to vibrations. Here, we present interface Self-Referenced (iSR) D-FFOCT, an alternative configuration to D-FFOCT that takes advantage of the presence of the sample coverslip in between the sample and the objective by using it as a defocused reference arm, thus avoiding the aforementioned artefacts. We demonstrate the ability of iSR D-FFOCT to image 2D fibroblast cell cultures, which are among the flattest mammalian cells.

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