Laboratory-based x-ray microtomography with directional dark-field sensitivity

We demonstrate dark-field x-ray microtomography in a compact, laboratory-based system capable of resolving attenuation, phase, and anisotropic scattering signals with micrometer-scale resolution across centimetre-scale samples. The method is based on two-directional beam tracking (2DBT), which requires only a single optical element and is compatible with standard x-ray sources and detectors. We validate the system's capabilities through imaging of a custom-built phantom, a fibre-reinforced composite and ex-vivo biological tissues, including a bovine intervertebral disc, a rat heart, and a porcine meniscus. The results show that dark-field tomography provides complementary information to attenuation as well as to phase tomography, by revealing sub-resolution features such as fibre orientation and microstructural heterogeneity at length scales that are well below the voxel size. A key element of our system is its sensitivity to scattering along two orthogonal directions in the image plane, enabling the measurement of scattering anisotropy with a single exposure. As well as simple and robust, our approach is sensitive and precise. These findings demonstrate the potential of 2DBT for non-destructive and three-dimensional structural characterisation of samples and materials in engineering, materials science and biomedical applications.