Quantification of the Removal and Inactivation of Virus Particles

Researchers from the Netherlands have developed and demonstrated a new method of quantifying and characterizing antiviral properties of polymer-functionalized surfaces for virus filtration and inactivation. Specifically, a polyethylenimine (PEI)-coated poly(ether sulfone) (PES) micro-filtration membrane was used to adsorb, inactivate, as well as disassemble virus capsids. Using fluorescence microscopy, spectroscopy, and single particle counting, only a small fraction (1%) of intact viruses can pass through the membrane, and a large fraction of viruses became inactivated and disassembled. Not only does the virus adsorb onto the PEI coating, it also interacts with PEI to disassemble the virus capsid. This new method provides a simpler and faster quantification and characterization technique for virus filtration and inactivation in the medical and biological world.