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Super-resolution capacity of variance-based stochastic fluorescence microscopy

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Version 2 2024-01-17, 17:00
Version 1 2023-11-28, 17:00
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posted on 2024-01-17, 17:00 authored by Simon Labouesse, Jérôme Idier, Marc Allain, Guillaume Giroussens, Thomas Mangeat, Anne Sentenac
Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random Illumination Microscopy (RIM), forms the super-resolvedimage from the variance of images obtained with random speckled illuminations. However, thevalidity of this process has not been fully theorized. In this work, we characterize mathematicallythe sample information contained in the variance of diffraction-limited speckled images as a functionof the statistical properties of the illuminations. We show that an unambiguous two-fold resolutiongain is obtained when the speckle correlation length coincides with the width of the observationpoint spread function. Last, we analyze the difference between the variance-based techniques usingrandom speckled illuminations (as in RIM) and those obtained using random fluorophore activation(as in Super-resolution Optical Fluctuation Imaging, SOFI).

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