Two-step excitation of fluorescent proteins with real intermediary
  states

Wong-Campos, J. David; Ornelas-Huerta, Dalia P.; Dion, Mackenzie

doi:10.48550/arXiv.2503.18000

Two-step excitation of fluorescent proteins with real intermediary states

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posted on 2025-03-26, 16:01 authored by J. David Wong-Campos, Dalia P. Ornelas-Huerta, Mackenzie Dion

We demonstrate sequential two-photon fluorescence microscopy using forbidden state transitions. Nonlinear red excitation leads to green fluorescence in live cells expressing eYFP, maintaining optical sectioning and allowing deep tissue imaging with simple optical systems.

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URL - References https://doi.org/10.48550/arXiv.2503.18000

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Two-step excitation of fluorescent proteins with real intermediary states

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